联系我们/CONTACT US

联系人：钱经理

电话：13636351217

手机：13636351217，13636351073

地址：上海市松江临港科技城汉桥文化科技园B座

邮编：201615

传真：021-64881400

邮箱：2881726255@qq.com

阿仪网商铺：http://www.app17.com/c60514/

手机网站：m.shhyswkj.com

产品分类/PRODUCT CLASS

技术文章

恒远产品文献: 小鼠8羟基脱氧鸟苷,小鼠过氧化脂质ELISA试剂盒引用文献

点击次数：1883 发布时间：2022/2/15 10:53:22

恒远产品文献: 小鼠8羟基脱氧鸟苷,小鼠过氧化脂质ELISA试剂盒引用文献
【文献标题】Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation
【作者】Bing Liu，Jun-Li Wang，Xin-Mei Wang，et.al
【作者单位】北京农业大学（Beijing University of Agriculture）
【文献中引用产品】
小鼠8羟基脱氧鸟苷（8-OHdG）ELISA试剂盒
小鼠过氧化脂质（LPO）ELISA试剂盒
【关键词】Superovulatory times，Injury model，LBP，Remedy，Oxidative stress
【DOI】https://doi.org/10.1016/j.theriogenology.2020.01.048
【出版期刊】《Theriogenology》
【产品原文引用】
8-OHdG ａnd LPO levels of the collected ovarian tissues were determined using an ELISA kit, according to the manu-facturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd,Shanghai, China). The collected mouse ovaries were cracked with lysate. Each 10 mL henylmethane-sulfonyl (PMSF) was added in 1 mL radio immuno precipitation assay (RIPA) as lysate. Every 1 g of tissue with 9 mL lysate, then coated with mouse antibody against 8-OHdG or LPO. After incubation ａnd washing concentrations of 8-OHdG or LPO were determined as described previously .Intra-and inter-CVs: 8-OHdG (6.3%, 8.1%), LPO (5.2%, 7.4%).

原创作者：上海恒远生物技术发展有限公司