先让我们热烈恭贺中国农业科学院的老师成功发表文献。老师之订购了恒远生物高敏ELISA试剂盒，使用了我们的产品在《Science of the Total Environment》杂志上发表了文献——Assessing the bioavailability ａnd biotoxicity of spiromesifen ａnd its main metabolite spiromesifen-enol (M01) reveals the defense mechanisms of earthworms (Eisenia fetida) IF影响因子高达10.75分！恒远生物在此也非常感谢老师对我们信任与支持，希望通过我们的不懈努力能为科研事业做出更大的贡献！

这篇文献非常值得研读，其中的很多实验方法和思路值得我们去学习，一篇文献的诞生要费很多精力和心血，老师在做实验的过程中也遇到了很多难题，我们的技术人员也尽力提供帮助，互相学习互相进步。小编想要把这篇文献分享给大家，希望能够对做科研的小伙伴们有一点点帮助，也希望大家积的参与我们的“发论文赢奖学金”活动，我等着你们的投稿哦，下一个奖学金的说不定就是你！

【文献标题】Assessing the bioavailability ａnd biotoxicity of spiromesifen ａnd its main metabolite spiromesifen-enol (M01) reveals the defense mechanisms of earthworms (Eisenia fetida)

【作者】Jianwei Fang , Binning Wang , Kuan Fang ,et.al

【作者单位】中国农业科学院（ Chinese Academy of Agricultural Sciences）

【文献中引用产品】
蚯蚓超氧化物歧化酶（SOD）ELISA试剂盒活性

蚯蚓过氧化氢酶（CAT）ELISA试剂盒活性

蚯蚓丙二醛（MDA）ELISA试剂盒

【关键词】UPLC-MS/MS,Transcriptome sequencing,SEM,TEM,Common DEGs

【DOI】doi.org/10.1016/j.scitotenv.2021.151910

【影响因子(IF)】10.75

【出版期刊】《Science of the Total Environment》

【产品原文引用】
2.6. Determination of oxidative ａnd immune stress effects
The protein content was determined according to the manufacturer＇s instructions using a protein assay kit (Solarbio; China). The reaction between Coomassie brilliant blue ａnd protein was monitored at 595 nm, ａnd the protein content was determined based on the change in absorbance. The ·OH? content, SOD activity, CAT activity, ａnd MDA content were measured using an ELISA kit (Shanghai Hengyuan Biotechnology Co., Ltd.; China). The contents or activities were determined according to the changes in the absorbance induced by the color reaction.

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